High-parameter flow cytometry, immunohistochemistry (cryosections), bone marrow chimera techniques, DNA immunoblots, transwell assays, ELISA, PCR, qRT-PCR, western blotting, plasmid cloning, MACS and FACS cell separation, CNS immune cell isolation, antigen presentation assays, tetramer staining, primary murine cell culturing techniques, mouse handling and injections (i.v., i.p., SQ)
Flow cytometry, confocal microscopy, imaging cytometry, plasmid cloning, lentiviral infections/CRISPR knockdown, protein purification, Western blotting, qPCR, MACS cell separation, antigen-specific CTL sorting and expansion, CTL activation assays, cross-presentation assays.
Flow cytometry, Western blotting, ELISA, ImageStream, qRT-PCR, Immunofluorescence microscopy, Lysosome pH measurements, Retroviral vectors, Viability assays, Coimmunoprecipitation, CTL killing assays, Proliferation assays, Cell cycle analysis
Flow cytometry, confocal microscopy, western blot, recombinant protein purification, ELISA, PCR, RT-qPCR, colonic biopsy-based wounding assisted by a colonocope, in vitro cell culture, in vivo colitis modeling, murine necropsy
flow cytometry, FACS, AOM/DSS colon cancer mouse model, germ-free mice manipulation, T cell activation assays, microbiome analysis (mothur), anaerobic bacteria cultivation, colon immune cell isolation