RT-PCR Western blotting Cell culture and differentiation - primary and cell lines, fibroblasts, macrophages, and colon epithelial cells Confocal microscopy LTQ mass spectrometry HPLC Mouse models of obesity and insulin resistance Computational modeling (DOCK 4)
Multiplex bead-based assays, Flow cytometry and FACS sorting, Illumina-based sequencing (MiSeq), PCR, RT-PCR and QPCR, ELISpot and ELISA analyses, Tissue culture, small animal necropsy, DNA/RNA isolation and processing, ChIP, ATAC-seq
My research interests involve to investigate the molecular and cellular metabolic pathways in innate T cells and how they are different from conventional T cells. In our laboratory, we have PLZF tg mice in which CD4 T cells behaves like NKT cells, therefore, I am going to demonstrate the similarities in PLZF tg CD4 and innate NKT cells. Till now, we have demonstrated that PLZF tg CD4 T cells produce more ROS similar to NKT cells and produce more cytokines without activation. I have also demonstrated that PLZF tg CD4 T cells as compared to conventional CD4 from WTB6 mice, have less glucose...
Immunological Techniques- Flow cytometry, FACS sorting, Flowjo analysis, ELISA analyses.
Molecular Techniques- Real-time quantitative RT-PCR, Western Blot analysis, Cell death analysis
Microbiology techniques-- Bacterial isolation and identification, Cell culture, in-vitro Cell culture, Bone marrow macrophages and neutrophils isolation and culture Microscopy- Fluorescence microscopy, confocal microscopy Animal handling, genetic fingerprinting, Statistical analysis of the data.
Flow cytometry, histology, cell culture, cell sorting, microscopy, confocal microscopy, large animal necropsy, small animal necropsy, rodent survival surgery, PCR, qPCR, nucleic acid extraction, general molecular biology.
Multiplex bead-based assays, Flow cytometry and FACS sorting, Illumina-based sequencing (MiSeq), Sequencing data analysis using Mothur, Real-time quantitative RT-PCR, ELISpot and ELISA analyses, Western Blotting, Basic microbiology techniques, Tissue culture, Extensive cloning experience, Vaccine development
• Flowcytometry (Calibur, Canto, LSRII): intra/extracellular stainings and FlowJo analysis
• Cytokine detection: ELISA and Luminex
• In vitro assays: murine and human cells (B cells/T cells/PBMCs/DCs/primary bronchial epithelial cells) and cell isolation (PBMCs) or purification using MACS beads or sorting
• Epithelial barrier function assays: ECIS, T.E.E.R, permeability assays, cigarette smoke and rhinovirus-induced model
• Confocal microscopy
• Cell death assays, proliferation assays
• Molecular techniques: qPCR (Bio-Rad), Western blot
Cell culture (primary and cell lines), Western blot, ELISA, Immunohistochemical / immunofluorescent staining, RNA isolation, cDNA synthesis, qRT-PCR, siRNA transfection, shRNA nucleofection.
My research interests have been to identify the key elements for chemotherapeutic resistance in ovarian cancer microenvironment at both molecular and cellular levels. I explore the impact of fibroblasts and immune cells on cisplatin resistance and sensitivity in ovarian cancer cells and identify that effector T cells abrogate fibroblasts-mediated cisplatin resistance. This finding provides a scientific rationale for the combination of chemotherapy and immunotherapy.
Flow cytometry, tissue culture, Western Blot, qPCR, ChIP-PCR