Postdoctoral Fellows

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(Includes Name, Title, Research Interests, Techniques)
Research Fellow, Internal Medicine, Division of Rheumatology
Techniques:

RT-PCR Western blotting Cell culture and differentiation - primary and cell lines, fibroblasts, macrophages, and colon epithelial cells Confocal microscopy LTQ mass spectrometry HPLC Mouse models of obesity and insulin resistance Computational modeling (DOCK 4)

Immunology Mentor:
Joseph Holoshitz, M.D.
Research Fellow, Microbiology and Immunology
Research Interests:

My research interests involve to investigate the molecular and cellular metabolic pathways in innate T cells and how they are different from conventional T cells. In our laboratory, we have PLZF tg mice in which CD4 T cells behaves like NKT cells, therefore, I am going to demonstrate the similarities in PLZF tg CD4 and innate NKT cells. Till now, we have demonstrated that PLZF tg CD4 T cells produce more ROS similar to NKT cells and produce more cytokines without activation. I have also demonstrated that PLZF tg CD4 T cells as compared to conventional CD4 from WTB6 mice, have less glucose...

Techniques:

Immunological Techniques- Flow cytometry, FACS sorting, Flowjo analysis, ELISA analyses.

Molecular Techniques- Real-time quantitative RT-PCR, Western Blot analysis, Cell death analysis

Microbiology techniques-- Bacterial isolation and identification, Cell culture, in-vitro Cell culture, Bone marrow macrophages and neutrophils isolation and culture Microscopy- Fluorescence microscopy, confocal microscopy Animal handling, genetic fingerprinting, Statistical analysis of the data.

Immunology Mentor:
Cheong-Hee Chang, Ph.D.
Research Fellow, Internal Medicine - Division of Rheumatology
Techniques:

Cell culture (primary and cell lines), Western blot, ELISA, Immunohistochemical / immunofluorescent staining, RNA isolation, cDNA synthesis, qRT-PCR, siRNA transfection, shRNA nucleofection.

Immunology Mentor:
Joseph Holoshitz, M.D.
Research Fellow
Research Interests:

My research interests have been to identify the key elements for chemotherapeutic resistance in ovarian cancer microenvironment at both molecular and cellular levels. I explore the impact of fibroblasts and immune cells on cisplatin resistance and sensitivity in ovarian cancer cells and identify that effector T cells abrogate fibroblasts-mediated cisplatin resistance. This finding provides a scientific rationale for the combination of chemotherapy and immunotherapy.

Techniques:

Flow cytometry, tissue culture, Western Blot, qPCR, ChIP-PCR

Immunology Mentor:
Weiping Zou, M.D., Ph.D.
Research Fellow, Microbiology and Immunology
Immunology Mentor:
Malini Raghavan, Ph.D.