Postdoctoral Fellows

a b c d e f g h i j k l m n o p q r s t u v w x y z All
(Includes Name, Title, Research Interests, Techniques)
Research Fellow, Internal Medicine, Division of Rheumatology
Techniques:

RT-PCR Western blotting Cell culture and differentiation - primary and cell lines, fibroblasts, macrophages, and colon epithelial cells Confocal microscopy LTQ mass spectrometry HPLC Mouse models of obesity and insulin resistance Computational modeling (DOCK 4)

Immunology Mentor:
Joseph Holoshitz, M.D.
Research Fellow, Internal Medicine - Pulmonary/Critical Care
Immunology Mentor:
Marc Peters-Golden, M.D.
Research Fellow, Neurology
Techniques:

Multiplex bead-based assays, Flow cytometry and FACS sorting, Illumina-based sequencing (MiSeq), PCR, RT-PCR and QPCR, ELISpot and ELISA analyses, Tissue culture, small animal necropsy, DNA/RNA isolation and processing, ChIP, ATAC-seq

Immunology Mentor:
Benjamin Segal, M.D.
Research Fellow, Microbiology and Immunology
Research Interests:

My research interests involve to investigate the molecular and cellular metabolic pathways in innate T cells and how they are different from conventional T cells. In our laboratory, we have PLZF tg mice in which CD4 T cells behaves like NKT cells, therefore, I am going to demonstrate the similarities in PLZF tg CD4 and innate NKT cells. Till now, we have demonstrated that PLZF tg CD4 T cells produce more ROS similar to NKT cells and produce more cytokines without activation. I have also demonstrated that PLZF tg CD4 T cells as compared to conventional CD4 from WTB6 mice, have less glucose...

Techniques:

Immunological Techniques- Flow cytometry, FACS sorting, Flowjo analysis, ELISA analyses.

Molecular Techniques- Real-time quantitative RT-PCR, Western Blot analysis, Cell death analysis

Microbiology techniques-- Bacterial isolation and identification, Cell culture, in-vitro Cell culture, Bone marrow macrophages and neutrophils isolation and culture Microscopy- Fluorescence microscopy, confocal microscopy Animal handling, genetic fingerprinting, Statistical analysis of the data.

Immunology Mentor:
Cheong-Hee Chang, Ph.D.
Research Fellow, Internal Medicine - Division of Pulmonary and Critical Care Medicine
Immunology Mentor:
Marc Peters-Golden, M.D.
Research Fellow, Internal Medicine - Division of Infectious Diseases
Techniques:

In vitro culture of primary human cells, qRT-PCR, ELISA, Western blot, lentiviral transduction

Immunology Mentor:
Kathleen Collins, M.D., Ph.D.
Research Fellow, Pediatrics and Communicable Diseases
Immunology Mentor:
Carey Lumeng, M.D., Ph.D.
Research Fellow, Internal Medicine - Division of Rheumatology
Techniques:

Molecular biology techniques, PCRs/real-time qPCRs, western blotting, immunofluorescence/immunocytochemistry, cell culture

Immunology Mentor:
Joseph Holoshitz, M.D.
Research Fellow, Internal Medicine - Division of Pulmonary and Critical Care Medicine
Techniques:

Flow cytometry, histology, cell culture, cell sorting, microscopy, confocal microscopy, large animal necropsy, small animal necropsy, rodent survival surgery, PCR, qPCR, nucleic acid extraction, general molecular biology.

Immunology Mentor:
John Osterholzer, M.D.
Research Fellow, Internal Medicine - Division of Hematology and Oncology
Techniques:

Multiplex bead-based assays, Flow cytometry and FACS sorting, Illumina-based sequencing (MiSeq), Sequencing data analysis using Mothur, Real-time quantitative RT-PCR, ELISpot and ELISA analyses, Western Blotting, Basic microbiology techniques, Tissue culture, Extensive cloning experience, Vaccine development

Immunology Mentor:
Grace Chen, M.D., Ph.D.
Research Fellow, Internal Medicine
Techniques:

• Flowcytometry (Calibur, Canto, LSRII): intra/extracellular stainings and FlowJo analysis
• Cytokine detection: ELISA and Luminex
• In vitro assays: murine and human cells (B cells/T cells/PBMCs/DCs/primary bronchial epithelial cells) and cell isolation (PBMCs) or purification using MACS beads or sorting
• Epithelial barrier function assays: ECIS, T.E.E.R, permeability assays, cigarette smoke and rhinovirus-induced model
• Confocal microscopy
• Cell death assays, proliferation assays
• Molecular techniques: qPCR (Bio-Rad), Western blot
• Animal...

Research Fellow, Internal Medicine - Division of Rheumatology
Techniques:

Cell culture (primary and cell lines), Western blot, ELISA, Immunohistochemical / immunofluorescent staining, RNA isolation, cDNA synthesis, qRT-PCR, siRNA transfection, shRNA nucleofection.

Immunology Mentor:
Joseph Holoshitz, M.D.
Research Fellow
Research Interests:

My research interests have been to identify the key elements for chemotherapeutic resistance in ovarian cancer microenvironment at both molecular and cellular levels. I explore the impact of fibroblasts and immune cells on cisplatin resistance and sensitivity in ovarian cancer cells and identify that effector T cells abrogate fibroblasts-mediated cisplatin resistance. This finding provides a scientific rationale for the combination of chemotherapy and immunotherapy.

Techniques:

Flow cytometry, tissue culture, Western Blot, qPCR, ChIP-PCR

Immunology Mentor:
Weiping Zou, M.D., Ph.D.
Research Fellow, Microbiology and Immunology
Immunology Mentor:
Malini Raghavan, Ph.D.