RT-PCR Western blotting Cell culture and differentiation - primary and cell lines, fibroblasts, macrophages, and colon epithelial cells Confocal microscopy LTQ mass spectrometry HPLC Mouse models of obesity and insulin resistance Computational modeling (DOCK 4)
My research interests involve to investigate the molecular and cellular metabolic pathways in innate T cells and how they are different from conventional T cells. In our laboratory, we have PLZF tg mice in which CD4 T cells behaves like NKT cells, therefore, I am going to demonstrate the similarities in PLZF tg CD4 and innate NKT cells. Till now, we have demonstrated that PLZF tg CD4 T cells produce more ROS similar to NKT cells and produce more cytokines without activation. I have also demonstrated that PLZF tg CD4 T cells as compared to conventional CD4 from WTB6 mice, have less glucose...
Immunological Techniques- Flow cytometry, FACS sorting, Flowjo analysis, ELISA analyses.
Molecular Techniques- Real-time quantitative RT-PCR, Western Blot analysis, Cell death analysis
Microbiology techniques-- Bacterial isolation and identification, Cell culture, in-vitro Cell culture, Bone marrow macrophages and neutrophils isolation and culture Microscopy- Fluorescence microscopy, confocal microscopy Animal handling, genetic fingerprinting, Statistical analysis of the data.
Cell culture (primary and cell lines), Western blot, ELISA, Immunohistochemical / immunofluorescent staining, RNA isolation, cDNA synthesis, qRT-PCR, siRNA transfection, shRNA nucleofection.
My research interests have been to identify the key elements for chemotherapeutic resistance in ovarian cancer microenvironment at both molecular and cellular levels. I explore the impact of fibroblasts and immune cells on cisplatin resistance and sensitivity in ovarian cancer cells and identify that effector T cells abrogate fibroblasts-mediated cisplatin resistance. This finding provides a scientific rationale for the combination of chemotherapy and immunotherapy.
Flow cytometry, tissue culture, Western Blot, qPCR, ChIP-PCR