Sergey Seregin, PhD
I’m a motivated and energetic young scientist who received his Ph.D in Microbiology and Molecular Genetics from Michigan State University in 2010 (advisor: Andrea Amalfitano). I'm focused on my main goal: to develop a competitive research program in an independent extramurally funded research laboratory to help with important public health problem. My short-term goals are to keep actively publishing research papers, try to obtain my own independent research funding (K22, upon completion current F32), become an active member in the professional societies: American Association of Immunologists (AAI) and American Gastroenterological Association (AGA). My specialty is evaluating and modifying innate and adaptive immune responses in animal models of infections diseases (MCMV, Listeria monocytogenes, Clostridium difficile) and chemically-induced colitis and identifying functions of specific populations of immune cells in mediating these immune responses (NK, CD8+ T, NKT cells, Ly6C-hi inflammatory monocytes, neutrophils).
I'm interested in understanding how the host immune system and the gut microbiota modulate intestinal inflammation and carcinogenesis using mouse models. Specifically, we have focused on understanding the mechanism by which certain members of the Nod-like receptor (NLR) family that are largely involved in the sensing of bacteria or tissue damage protect against the development of inflammation-induced colitis and colon tumorigenesis. We are also investigating how the gut microbiota regulates colon tumorigenesis using germfree and gnotobiotic mice as well as attempting to identify tumor-suppressive and tumor-promoting bacterial populations through bacterial sequencing and analysis of stool from wildtype and NLR-deficient mice during the development of chemically-induced inflammation and tumors.
Multiplex bead-based assays, Flow cytometry and FACS sorting, Illumina-based sequencing (MiSeq), Sequencing data analysis using Mothur, Real-time quantitative RT-PCR, ELISpot and ELISA analyses, Western Blotting, Basic microbiology techniques, Tissue culture, Extensive cloning experience, Vaccine development
1. Seregin SS, Chen GY, Laouar Y. Dissecting CD8+ NKT Cell Responses to Listeria Infection Reveals a Component of Innate Resistance. J Immunol. 2015; 195:1112-1120. (0 citations).
2. Aldhamen YA, Pepelyayeva Y, Rastall DP, Seregin SS, et al. Autoimmune Disease-Associated Variants of Extracellular Endoplasmic Reticulum Aminopeptidase 1 Induce Altered Innate Immune Responses by Human Immune Cells. J Innate Immun. 2014;7:275-289. (1 citations).
3. Rastall DP, Aldhamen YA, Seregin SS, Godbehere S, Amalfitano A. ERAP1 functions override the intrinsic selection of specific antigens as immunodominant peptides, thereby altering the potency of antigen-specific cytolytic and effector memory T-cell responses. Int Immunol. 2014; 26:585-595. (2 citations).
4. Koestler BJ, Seregin SS, Rastall DP, et al. Stimulation of Innate Immunity by in vivo cyclic di-GMP Synthesis using Adenovirus. Clin Vaccine Immunol. 2014; 21:1550-1559. (0 citations).
5. Aldhamen YA, Seregin SS, Aylsworth CF, Godbehere S, Amalfitano A. Manipulation of EAT-2 expression promotes induction of multiple beneficial regulatory and effector functions of the human innate immune system as a novel immunomodulatory strategy. Int Immunol. 2013; 26(5):291-303. (1 citations).
6. Zervoudi E, Saridakis E, Birtley JR, Seregin SS, et al. Rationally designed inhibitor targeting antigen-trimming aminopeptidases enhances antigen presentation and cytotoxic T-cell responses. Proc Natl Acad Sci U S A. 2013;110:19890-19895. (18 citations).
7. Seregin SS, Rastall DP, Evnouchidou I, et al. Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens. Autoimmunity. 2013;46:497-508. (17 citations).
8. Kovalenko PL, Yuan L, Sun K, Seregin SS, et al. Regulation of epithelial differentiation in rat intestine by intraluminal delivery of an adenoviral vector or silencing RNA coding for schlafen 3. PLoS One. 2013;8:e79745. (3 citations).
9. Aylsworth CF, Aldhamen YA, Seregin SS, Godbehere S, Amalfitano A. Activation of human natural killer cells by the novel innate immune modulator recombinant Eimeria antigen. Hum Immunol. 2013;74:916-926. (4 citations).
10. Seregin SS, Aldhamen YA, Rastall DP, et al. Endoplasmic reticulum aminopeptidase-1 functions regulate key aspects of the innate immune response. PLoS One. 2013;8:e69539. (7 citations).