Sarah Veloso Nogueira, PhD

Research Fellow, Internal Medicine - Division of Rheumatology

Biography

I did all my education in Brazil, which is where I am from. I completed my undergraduate at Federal University of Goias with a bachelor in Pharmacy, and I worked with plant antibiotics activities upon E. coli strains. Following my undergraduate, I worked with Cowpox virus analyzing signal transduction pathways in cells infected with this virus during Masters. For my PhD in Microbiology, I have worked with the pathogenic fungus Paracoccioides brasiliensis, specifically with a protein involved in adhesion to extracellular matrix proteins, plasminogen and host cells. I have identified this protein after sequencing differential products from a comparative transcriptome analysis (RDA). In a previous postdoctoral position, I was studying a protein involved in adhesion and pathogenesis in Borrelia burgdorferi and Leptospira interrogans. After that I worked for 2 years in a lab at Federal University of Goias and contributed with 2 publications.

Research Interests

Dr Holoshitz's lab is interested in the underlying mechanisms by which the Shared Epitope (SE) affects susceptibility to, or the severity of, Rheumatoid arthritis. My project is focused on identifying genes regulated by SE in RAW cells.

Techniques

Molecular biology techniques, PCRs/real-time qPCRs, western blotting, immunofluorescence/immunocytochemistry, cell culture

Publications

Da Costa AC, Costa-Junior A de O, de Oliveira FM, Nogueira SV, Rosa JD, Resende DP, Kipnis A, Junqueira-Kipnis AP. 2014. A new recombinant BCG vaccine induces specific Th17 and Th1 effector cells with higher protective efficacy against tuberculosis. PLOS ONE, 9(11):112848.

Da Costa A.C., Nogueira S.V., Kipnis A., Junqueira-Kipnis A.P. 2014. Recombinant BCG: Innovations on an old vaccine. Scope of BCG strains and strategies to improve long-lasting memory. Front Immunol. 7; 5:152.

Sarah Veloso Nogueira; Brian T. Backstedt; Alexis A. Smith; Jin-Hong Qin; Elsio A. Wunder Jr; Albert Ko; Utpal Pal. 2013. Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen. PLOS ONE, 8(10):e78150.

Nogueira S.V., Smith A. A., Qin, J. H., Pal. U. 2012. A surface enolase participates in Borrelia burgdorferi-plasminogen interaction and contributes to pathogen survival within feeding ticks. Infect. Immun., 80: 82-80.

Bailão A.M., Nogueira S.V., Bonfim S. M. R. C., Castro K. P., Silva J. F., Mendes-Giannini M. J., Pereira M., de Alimeida Soares C. M. 2012. Comparative transcriptome analysis of Paracoccidioides brasiliensis during in vitro adhesion to type I collagen and fibronectin: Identification of potential adhesins. Res. Microbiol., 163: 182- 91.

Nogueira S.V., Fonseca F. L., Rodrigues M. L., Mundodi V., Abi-Chacra E. A., Winters M. S., Alderete J. F., Soares C. M. A. 2010. Paracoccidioides brasiliensis enolase is a surface protein that binds plasminogen and mediates interaction of yeast forms with host cells. Infect. Immun., 78: 4040-50.

Donofrio, F. C., A. C. Calil, E. T. Miranda, A. M. Almeida, G. Benard, C. P. Soares, S. V. Nogueira, C. M. A. Soares, M. J. Mendes-Giannini. 2009. Enolase from Paracoccidioides brasiliensis: isolation and identification as a fibronectin-binding protein. J Med Microbiol 58: 706-13.

Sousa LP, Brasil BS, Silva Bde M, Nogueira SV, Andrade AA, Ferreira PC, Teixeira SM, Gollob KJ, Kroon EG, Kato K, Bonjardim CA. Characterization of alpha-enolase as an interferon-alpha 2 alpha 1 regulated gene. Front. Biosci., v.10, p.2534-47, 2005.

De Sousa LP, Brasil BS, Silva BM, Freitas MH, Nogueira SV, Ferreira PC, Kroon EG, Bonjardim CA. Plasminogen/plasmin regulates c-fos and egr-1 expression via the MEK/ERK pathway. Biochem. Biophys. Res. Commun., v. 1, p. 237-45, 2005.

Sousa LP, Silva BM, Brasil BS, Nogueira SV, Ferreira PC, Kroon EG, Kato K, Bonjardim CA. Plasminogen/plasmin regulates alpha-enolase expression through the MEK/ERK pathway. Biochem. Biophys. Res. Commun., v., 4, p. 1065-71, 2005.

Silva PN, Soares JA, Brasil BS, Nogueira SV, Andrade AA, de Magalhães JC, Bomjardim MB, Ferreira PC, Kroon EG, Bruna-Romero O, Bomjardim CA. Differential role played by the MEK/ERK/EGR-1 pathway in orthopoxviruses vaccinia and cowpox biology. Biochem. J., 2006.

Immunology Mentor